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  • 18-June-2021

    English

    Test No. 249: Fish Cell Line Acute Toxicity - The RTgill-W1 cell line assay

    The RTgill-W1 cell line assay describes a 24-well plate format fish cell line acute toxicity test using the permanent cell line from rainbow trout (Oncorhynchus mykiss) gill, RTgill-W1. After 24 h of exposure to the test chemical, cell viability is assessed based on three fluorescent cell viability indicator dyes, measured on the same set of cells. Resazurin enters the cells in its non-fluorescent form and is converted to the fluorescent product, resorufin, by mitochondrial, microsomal or cytoplasmic oxidoreductases. A reduction in the fluorescence of resorufin indicates a decline in cellular metabolic activity, including disruption of mitochondrial membranes. The data are expressed as the percent cell viability of unexposed control values versus the test chemical concentration. The resulting concentration-response curves serve to determine the effective concentrations causing 50% loss in cell viability, i.e. the EC50 value. The test is designed to (i) predict fish acute toxicity in product testing; (ii) range-finding and pre-screening before conducting a full fish acute or other fish-based toxicity test; (iii) generation of toxicity information to be used for hazard assessment in combination with other lines of evidences (e.g., Quantitative Structure Activity Relationships (QSAR), weight of evidence (WoE)) within Integrated Testing Strategy (ITS)/Integrated Approach to Testing and Assessment (IATA).
  • 17-June-2021

    English

    Towards a national strategic framework for the circular economy in the Czech Republic - Analysis and a proposed set of key elements

    The past decades have witnessed unprecedented growth in global consumption of raw materials, which also lead to increased environmental pressures. As a response to these challenges, countries across the world have made the transition to a circular economy one of their policy priorities. This report develops a set of key elements of a strategic framework for the circular economy in the Czech Republic, which the government is developing (Circular Czechia 2040). It discusses the rationale for transitioning to a circular economy in the Czech Republic and provides an in-depth analysis of four priority areas: consumption and consumers, waste management, economic instruments, as well as research innovation and digitalisation. The report also suggests 40 concrete policy measures to help implement the strategic framework, along with a monitoring framework to measure the progress made. This report was co-funded by the European Union via the Structural Reform Support Programme.
  • 17-June-2021

    English

    Test No. 498: In vitro Phototoxicity - Reconstructed Human Epidermis Phototoxicity test method

    Skin phototoxicity (photoirritation) is defined as an acute toxic response elicited by topically or systemically administered photoreactive chemicals after the exposure of the skin to environmental light. The in vitro reconstructed human epidermis phototoxicity test (RhE PT) is used to identify the phototoxic potential of a test chemical after topical application in reconstructed human epidermis (RhE) tissues in the presence and absence of simulated sunlight. Phototoxicity potential is evaluated by the relative reduction in viability of cells exposed to the test chemical in the presence as compared to the absence of simulated sunlight. Chemicals identified as positive in this test may be phototoxic in vivo following topical application to the skin, eyes, and other external light-exposed epithelia.
  • 17-June-2021

    English

    Test No. 455: Performance-Based Test Guideline for Stably Transfected Transactivation In Vitro Assays to Detect Estrogen Receptor Agonists and Antagonists

    This Performance-Based Test Guideline (PBTG) describes in vitro assays, which provide the methodology of Stably Transfected Transactivation to detect Estrogen Receptor Agonists and Antagonists (ER TA assays). It comprises mechanistically and functionally similar test methods for the identification of estrogen receptor agonists and antagonists and should facilitate the development of new similar or modified test methods. The two reference test methods that provide the basis for this PBTG are: the Stably Transfected TA (STTA) assay using the (h) ERα-HeLa-9903 cell line, derived from a human cervical tumor, and the BG1Luc ER TA assay using the BG1Luc-4E2 cell line, derived from a human ovarian adenocarcinoma. The cell lines used in these assays express ER and have been stably transfected with an ER responsive luciferase reporter gene. The assays are used to identify chemicals that activate (i.e. act as agonists) and also suppress (i.e. act as antagonists) ER- dependent transcription. ER are activated following ligand binding, after which the receptor-ligand complex binds to specific DNA response elements and transactivates the reporter gene, resulting in increased cellular expression of a marker enzyme (e.g. luciferase in luciferase based systems). The enzyme then transforms the substrate to a bioluminescent product that can be quantitatively measured with a luminometer. These test methods are being proposed for screening and prioritisation purposes, but also provide mechanistic information that can be used in a weight of evidence approach.
  • 17-June-2021

    English

    Test No. 250: EASZY assay - Detection of Endocrine Active Substances, acting through estrogen receptors, using transgenic tg(cyp19a1b:GFP) Zebrafish embrYos

    The EASZY assay is a mechanism-based in vivo screening assay designed to detect endocrine active chemicals acting as agonist through estrogen receptors (ERs), by inducing the expression of the green fluorescent protein (GFP) driven by the cyp19a1b promoter. The EASZY assay allows for the detection of estrogenic activity of chemicals on transgenic tg(cyp19a1b:GFP) Zebrafish embryos exposed for 96 hours during the embryonic stages of development. At the end of the experiment, the fluorescence of each newly hatched eleutheroembryo is measured using fluorescence microscope. Because the skull of early developmental stages of zebrafish is transparent, GFP is observed, imaged and quantified in vivo. The intensity of fluorescence is then quantified using image analysis software.
  • 17-June-2021

    English

    Test No. 509: Crop Field Trial

    Crop field trials are conducted to determine the magnitude of the pesticide residue in or on raw agricultural commodities, including feed items, and should be designed to reflect pesticide use patterns that lead to the highest possible residues. Objectives of crop field trials are to: (1) quantify the expected range of residue(s) in crop commodities following treatment according to the proposed or established good agricultural practice; (2) determine, when appropriate, the rate of decline of the residue(s) of plant protection product(s) on commodities of interest; (3) determine residue values such as the 'Supervised Trial Median Residue' and 'Highest Residue' for conducting dietary risk assessment; and (4) derive maximum residue limits (MRLs).  This Test Guideline requires one sample from treated plots at each sampling interval for crops that have eight or more crop field trials. The test substance(s) should be stored under appropriate conditions for the study duration and applied soon after preparation or mixing. Test substance applications should not be made in strong wind, during rain or when rainfall is expected shortly after application. For all applications, the application rate should be expressed in terms of amount of product and/or active ingredient per unit area. At the end of each crop field trial, the (stored) samples are analysed for residue level (expressed for example in mg/kg).
  • 17-June-2021

    English

    Test No. 405: Acute Eye Irritation/Corrosion

    This method provides information on health hazard likely to arise from exposure to test substance (liquids, solids and aerosols) by application on the eye. This Test Guideline is intended preferably for use with albino rabbit. The test substance is applied in a single dose in the conjunctival sac of one eye of each animal. The other eye, which remains untreated, serves as a control. The initial test uses an animal; the dose level depends on the test substance nature. A confirmatory test should be made if a corrosive effect is not observed in the initial test, the irritant or negative response should be confirmed using up to two additional animals. It is recommended that it be conducted in a sequential manner in one animal at a time, rather than exposing the two additional animals simultaneously. The duration of the observation period should be sufficient to evaluate fully the magnitude and reversibility of the effects observed. The eyes should be examined at 1, 24, 48, and 72 hours after test substance application. The ocular irritation scores should be evaluated in conjunction with the nature and severity of lesions, and their reversibility or lack of reversibility. Use of topical anesthetics and systemic analgesics to avoid or minimize pain and distress in ocular safety testing procedures is described.
  • 11-June-2021

    English

    The OECD Control System for waste recovery

    An update of the OECD database on transboundary movements of wastes is now available for download. Since 1992, transboundary movements of recyclable wastes between OECD countries are regulated by the Council Decision C(2001)107, which was established by the OECD Council, and designed as an agreement under Article 11 of the Basel Convention.

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  • 10-June-2021

    English

    OECD Workshop on Regional Trade Agreements and the Environment, 10-11 June 2021

    10-11 June 2021 - This workshop focused on three objectives: (i) to take stock of current experience and insights on RTAs and the environment from stakeholders, (ii) to investigate how RTAs can serve as a vehicle to advance a resource efficient and circular economy transition, and (iii) to explore the potential of RTAs in addressing the nexus of illegal trade and environmental crime.

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  • 9-June-2021

    English

    Customisation Opportunities of IUCLID for the Management of Chemical Data – 2nd edition

    IUCLID (International Uniform Chemical Information Database) is a software application designed to record, store, maintain and exchange data on chemicals. It is a key software application for both regulatory bodies and the chemical industry where it is used in the implementation of various regulatory programmes. IUCLID can be customised and configured to manage chemical data in different contexts and is a platform employing globally harmonised data elements pertinent to chemicals. It is continuously updated to provide greater customisation, extension and integration with other tools. This second edition provides the latest updates on IUCLID features and processes, including visual 'working' contexts for the preparation and management of data according to regulatory contexts or data processes, possibilities for data entry in multiple languages, and a matrix view of the use of IUCLID in OECD countries.
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