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Section 4: Software, Spreadsheets, Protocols and other TG supporting material

 

Disclaimer: the software package, spreadsheets or protocols available for some Test Guidelines are provided as a courtesy of the developer of the test method, but have not been necessarily validated, reviewed or approved by the OECD. It is also not a requirement to use this material, and other software for example may be equally useful. The maintenance of the software or of the calculation sheets is not guaranteed by the OECD. For use of the software under Good Laboratory Practice, the validation status should be checked and any additional validation performed as appropriate.

Example of a protocol for the Mammalian Erythrocyte Pig-a Gene Mutation Assay (TG 470)

This document is a pre-print from a book chapter on Erythrocyte-Based Pig-a Gene Mutation Assay (PDF)
Bemis JC, NE Hall, SD Dertinger (2019) Erythrocyte-based Pig-a gene mutation assay. In: A Dhawan, M Bajpayee (eds), Genotoxicity Assessment: Methods and Protocols, Methods in Molecular Biology, vol 2031, Humana, NY, NY. pp 29-57. Available at: https://doi.org/10.1007/978-1-4939-9646-9_2.

 

QSAR Model Reporting Format to be used with TG 497 (DASS)

This reporting format has been slightly adapted to meet the needs of the Guideline on Defined Approaches on Skin Sensitisation (Word)

 

Spreadsheets for use with TG 458

- log PC10, log PC50 and RPCmax for agonist assay: Spreadsheet agonist

- log IC50 and log IC30 for antagonist: Spreadsheet antagonist

 

Spreadsheet for use with kDPRA in TG 442C

This automated Excel-evaluation spreadsheet is used for data evaluation of the test methods described in Appendix III of TG 442C: Spreadsheets for TG 442C - Appendix III : The kinetic Direct Peptide Reactivity assay (kDPRA). It is also available with the DB-ALM protocol no. 217* which provides detailed instructions on how to use it.

*DB-ALM Protocol 217: The kinetic Direct Peptide Reactivity assay (kDPRA). Accessible at DB-ALM Protocol n° 217: Kinetic Direct Peptide Reactivity Assay (kDPRA) 

 

Software to be used with TG 455

For calculations of PC10, PC50 and PCMax for the STTA TG 455, an Excel spreadsheet is available. Please follow the instructions below.


- Download the Excel spreadsheet for the:

  • Agonist part of the assay (Excel)
  • Antagonist part of the assay (Excel)

- Follow the instruction manual (Link to the instruction manual of the TG 455 spreadsheet) (PDF)

- The Quality Assurance Statement is available from the Secretariat

 

Software to be used with TG 432: Phototox Version 2.0

To install the software download phototox.zip to the hard drive of your computer and then open the zip folder and click on the setup.exe file. The software will install itself on your computer.For instructions on how to use the PHOTOTOX Application please read the How-to-get-started document (Word, PDF) and the PHOTOTOX Application Help in the phototox.hlp file contained in the phototox.zip file.Other documentation for the software:

  • Phase II.PXP and Phase III.PXP (PXP project files containing all the validation data) (ZIP file)
  • In Vitro Phototoxicity Testing: Development and Validation of a New Concentration Response Analysis Software and Biostatistical Analyses Related to the Use of Various Prediction Models (PDF)

 

Software to be used with TG 425

  • Statistical Software to be used with TG425: Acute Oral Toxicity: Up and Down Procedure

User documentation 329k- PDF for the AOT425StatPgm Program - Users should read this file before downloading the AOT425 Statistical Program file

Next, download the AOT 425Statistical Program.This is a self-extracting zip file which will install the program on your computer in two steps (right click on link, then "Save target as...") 1.84mb- execute file.

Questions or comments? Email AOT425@epa.gov.

Note: This is to confirm that the use of the computer program, AOT425StatPgm, developed by Westat for the US EPA, is freely given and there are no licensing restrictions in connection with its use. The user is cautioned to read the User Documentation regarding any limitations on applicability and hardware requirements.

 

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