This document is a pre-print from a book chapter on Erythrocyte-Based Pig-a Gene Mutation Assay (PDF)
Bemis JC, NE Hall, SD Dertinger (2019) Erythrocyte-based Pig-a gene mutation assay. In: A Dhawan, M Bajpayee (eds), Genotoxicity Assessment: Methods and Protocols, Methods in Molecular Biology, vol 2031, Humana, NY, NY. pp 29-57. Available at: https://doi.org/10.1007/978-1-4939-9646-9_2.
This reporting format has been slightly adapted to meet the needs of the Guideline on Defined Approaches on Skin Sensitisation (Word)
- log PC10, log PC50 and RPCmax for agonist assay: Spreadsheet agonist
- log IC50 and log IC30 for antagonist: Spreadsheet antagonist
This automated Excel-evaluation spreadsheet is used for data evaluation of the test methods described in Appendix III of TG 442C: Spreadsheets for TG 442C - Appendix III : The kinetic Direct Peptide Reactivity assay (kDPRA). It is also available with the DB-ALM protocol no. 217* which provides detailed instructions on how to use it.
*DB-ALM Protocol 217: The kinetic Direct Peptide Reactivity assay (kDPRA). Accessible at DB-ALM Protocol n° 217: Kinetic Direct Peptide Reactivity Assay (kDPRA)
For calculations of PC10, PC50 and PCMax for the STTA TG 455, an Excel spreadsheet is available. Please follow the instructions below.
- Download the Excel spreadsheet for the:
- Follow the instruction manual (Link to the instruction manual of the TG 455 spreadsheet) (PDF)
- The Quality Assurance Statement is available from the Secretariat
To install the software download phototox.zip to the hard drive of your computer and then open the zip folder and click on the setup.exe file. The software will install itself on your computer.For instructions on how to use the PHOTOTOX Application please read the How-to-get-started document (Word, PDF) and the PHOTOTOX Application Help in the phototox.hlp file contained in the phototox.zip file.Other documentation for the software:
User documentation 329k- PDF for the AOT425StatPgm Program - Users should read this file before downloading the AOT425 Statistical Program file
Next, download the AOT 425Statistical Program.This is a self-extracting zip file which will install the program on your computer in two steps (right click on link, then "Save target as...") 1.84mb- execute file.
Questions or comments? Email AOT425@epa.gov.
Note: This is to confirm that the use of the computer program, AOT425StatPgm, developed by Westat for the US EPA, is freely given and there are no licensing restrictions in connection with its use. The user is cautioned to read the User Documentation regarding any limitations on applicability and hardware requirements.
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