Test No. 444A: In Vitro Immunotoxicity

This Test Guideline (TG) describes the IL-2 Luc and IL-2 Luc Leukocyte Toxicity Test (LTT)Assays to evaluate the potential immunotoxic effects of chemicals on T lymphoblastic cell line. This cell line allows quantitative measurement of luciferase gene induction by detecting luminescence from well-established light producing luciferase substrates as indicators of the activity of IL-2, IFN-γ and GAPDH in cells following exposure to immunotoxic chemicals. The method is intended to be used as a part of a battery to determine immunotoxic potential of chemicals.