A critical issue to consider when selecting a cell or tissue based test system is the source of the cells or tissues, as its history/handling may influence its characteristics and, consequently, the results of the in vitro methods conducted with this test system. Sourcing of cells and tissues from a certified provider, e.g., established cell banks with a high quality standard, commercial providers, or reputable culture collections, who usually provide extensive documentation on the origins and characterisation of the test system is recommended (see Guidance on Good In vitro Method practices, Chapter 5.2 [OECD, 2025]).
Deposition, availability and distribution of cell lines
Cell lines are a critical component of many OECD in vitro methods. Information is available on where these cell lines can be obtained and on practices that support their long-term availability and use.
Why cell line banking is important?
Where are the cell lines used in OECD Test Guidelines available?
For OECD Test Guidelines implementation in good conditions, users need to readily access cell lines referenced from cell banks, reputable commercial providers or cell culture collections. An inventory of cell lines and sources identified in Test Guidelines, and by cell banks themselves, is available from this file. Annual updates will be made in July-August on the basis of information in (new and updated) Test Guidelines and updates from cell banks.
What are the steps to deposit or obtain a cell line from a cell bank?
The OECD is collaborating with global cell banks and cell culture collections in member countries to develop some good practices for cell line deposition and for cell line procurement by testing facilities. As the collaboration progresses, existing practices will be shared on the basis of which good practices can be established and promoted to facilitate the implementation of in vitro methods in member and adhering countries.
