Validation of the Short‑term Juvenile Hormone Activity Screening Assay using Daphnia magna (JHASA)

This document presents the design and the results of a validation exercise for the short-term Juvenile Hormone Activity Screening Assay (JHASA) using Daphnia magna, organised by Japan. This method was developed to detect the potential endocrine disrupting chemicals (EDCs) with juvenile hormone activity. The project was on the Test Guidelines Programme work plan since 2016 and until 2024.