The glucocorticoid receptor (GR) is a hormone-activated transcription factor involved in a wide range of key physiological processes, including the regulation of stress response, immune function, inflammation, and metabolism. The proposed human GR transactivation assay enables detection of agonists (activation) and antagonist (inhibition) of the GR. It uses a cell line stably transfected with a luciferase reporter gene under the control of the GR responsive element to assess GR activity.
Draft Test Guideline on stably transfected human glucocorticoid receptor transcriptional activation assay
- Submission period
- 18 Dec 2025 - 5 Feb 2026
Background public consultation
Project description
The project to develop an OECD Test Guideline on the Stably transfected human glucocorticoid receptor (hGR) transcriptional activation assay for detection of agonistic and antagonist activity of chemicals towards hGR, was proposed by France and Canada and included in the Test Guidelines Programme workplan in 2022. The proposed draft Test Guideline was developed by the lead, with the support of an OECD Expert group, following completion of a validation study and the subsequent peer review of its results.
How to contribute
The draft human glucocorticoid receptor transcriptional activation assay is currently under a commenting round by the Working Party of the National Coordinators of the Test Guidelines Programmes. It is open for comments from the public at the same time. Interested parties are invited to contact their National Coordinators at least a week before 5 February 2026.
