Test No. 498: In vitro Phototoxicity - Reconstructed Human Epidermis Phototoxicity
test method
Skin phototoxicity (photoirritation) is defined as an acute toxic response elicited
by topically or systemically administered photoreactive chemicals after the exposure
of the skin to environmental light. The in vitro reconstructed human epidermis phototoxicity
test (RhE PT) is used to identify the phototoxic potential of a test chemical after
topical application in reconstructed human epidermis (RhE) tissues in the presence
and absence of simulated sunlight. Phototoxicity potential is evaluated by the relative
reduction in viability of cells exposed to the test chemical in the presence as compared
to the absence of simulated sunlight. Chemicals identified as positive in this test
may be phototoxic in vivo following topical application to the skin, eyes, and other
external light-exposed epithelia.
Published on June 14, 2021Also available in: French
In series:OECD Guidelines for the Testing of Chemicals, Section 4: Health Effectsview more titles