This method provides information on health hazard likely to arise from exposure to
liquid or solid test substance by dermal application. This Test Guideline recommends
sequential testing strategies, which include the performance of validated and accepted
in vitro or ex vivo tests for corrosion/irritation.The albino rabbit is the preferable
laboratory animal. The substance to be tested is applied in a single dose to a small
area of skin (approximately 6cm²) of an experimental animal; untreated skin areas
of the test animal serve as the control. The exposure period is 4 hours. Residual
test substance should then be removed. The dose is 0.5ml (liquid) or 0.5g (solid)
applied to the test site. The method consists of two tests: the initial test and the
confirmatory test (used only if a corrosive effect is not observed in the initial
test). All animals should be examined for signs of erythema and oedema during 14 days.
The dermal irritation scores should be evaluated in conjunction with the nature and
severity of lesions, and their reversibility or lack of reversibility. When responses
persist to the end of the 14-day observation period, the test substance should be
considered an irritant.