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This Test Guideline describes an assay that assesses early life-stage effects and potential adverse consequences of putative endocrine disrupting chemicals (e.g. oestrogens, androgens and steroidogenesis inhibitors) on fish sexual development. In the test, fish are exposed, from newly fertilized egg until the completion of sexual differentiation at about 60 days post hatch, to at least three concentrations of the test substance
This Test Guideline describes an in vitro screen for chemical effects on steroidogenesis, specifically the production of 17ß-estradiol (E2) and testosterone (T). The human H295R adreno-carcinoma cell line, used for the assay, expresses genes that encode for all the key enzymes for steroidogenesis. After an acclimation period of 24 h in multi-well plates, cells are exposed for 48 h to seven concentrations of the test chemical in at
The purpose of this test is to determine the effects of a substance on the growth of freshwater microalgae and/or cyanobacteria. Exponentially growing test organisms are exposed to the test substance in batch cultures over a period of normally 72 hours.
The system response is the reduction of growth in a series of algal cultures exposed to, at least, five concentrations of a test substance. Three replicates at each test
This Test Guideline describes an in vivo assay that detects chemicals that may induce gene mutations. In this assay, transgenic rats or mice that contain multiple copies of chromosomally integrated plasmid or phage shuttle vectors are used. The transgenes contain reporter genes for the detection of various types of mutations induced by test substances. A negative control group and a minimum of 3 treatment groups of
This Test Guideline describes an acute immobilisation assay on chronomids and is designed to complement existing Test Guidelines for chironomid chronic toxicity assays (TG 218, 219 and 233). The test method is based on TG 202: Daphnia sp. Acute Immobilisation Test. First instar Chironomus sp. larvae are exposed to a range of concentrations of the test substance in water-only vessels for a period of 48 hours. C. riparius is the
This Test Guideline is designed to provide an evaluation of reproductive and developmental effects that may occur as a result of pre- and postnatal chemical exposure as well as an evaluation of systemic toxicity in pregnant and lactating females and young and adult offspring. In the assay, sexually-mature males and females rodents (parental (P) generation) are exposed to graduated doses of the test substance starting 2 weeks before
The Pesticide Programme of the OECD looks to help governments co-operate in assessing and reducing the risks of agricultural pesticides. The OECD encourages governments to share the work of pesticide registration and develops tools to monitor and minimise pesticide risk to health and the environment.
The objective of the OECD Biocide Programme is to attain a harmonised approach in the regulation of biocides in all OECD countries. The OECD is thus working on harmonising the main data requirements for biocides and the methodologies for the interpretation of these data.
The focus of the Workshop was to show the various attempts to implement service-oriented strategies such as "Chemical Leasing" models in practice. Moreover the possible economic and environmental benefits as well as the possible obstacles and drawbacks should be elucidated. The conclusions were to give an assessment of the prospects of future development for such business models.
This is the third in a series of documents produced by the OECD as part of its work on socio-economic analysis. The other two are: Guidance for Conducting Retrospective Studies on Socio-Economic Analysis (1999) and Framework for Integrating Socio-Economic Analysis in Chemical Risk Management Decision Making (2000).