This method provides information on health hazard likely to arise from exposure to
test substance (liquids, solids and aerosols) by application on the eye. This Test
Guideline is intended preferably for use with albino rabbit. The test substance is
applied in a single dose in the conjunctival sac of one eye of each animal. The other
eye, which remains untreated, serves as a control. The initial test uses an animal;
the dose level depends on the test substance nature. A confirmatory test should be
made if a corrosive effect is not observed in the initial test, the irritant or negative
response should be confirmed using up to two additional animals. It is recommended
that it be conducted in a sequential manner in one animal at a time, rather than exposing
the two additional animals simultaneously. The duration of the observation period
should be sufficient to evaluate fully the magnitude and reversibility of the effects
observed. The eyes should be examined at 1, 24, 48, and 72 hours after test substance
application. The ocular irritation scores should be evaluated in conjunction with
the nature and severity of lesions, and their reversibility or lack of reversibility.
Use of topical anesthetics and systemic analgesics to avoid or minimize pain and distress
in ocular safety testing procedures is described.