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Testing of chemicals

Test No. 247: Bumblebee, Acute Oral Toxicity Test

In series:OECD Guidelines for the Testing of Chemicals, Section 2: Effects on Biotic Systemsview more titles

Published on October 09, 2017

Also available in: French

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This test guideline is a laboratory test method, designed to assess the acute oral toxicity of pesticides and other chemicals to adult worker bumblebees.Adult worker bumblebees are exposed to 50 % (w/v) aqueous sucrose solution containing the test chemical. The test duration is at least 48 h. Mortality is recorded daily and compared with control values. Results are analysed in order to calculate the LD50 and NOED, if possible, at 24 h & 48 h and furthermore at 72 h & 96 h in case the study is prolonged.