Testing of chemicals

OECD Test Guidelines for the Chemicals


What's new

On 18 June, OECD adopted a set of new and updated Test Guidelines for chemicals safety testing to keep pace with scientific progress for OECD members and Mutual Acceptance of Data (MAD) adhering countries.

Those include three new Test Guidelines:

Cover TG 494  

  • TG 494 describes a new in vitro method for eye irritation hazard, by identifying chemicals not requiring classification for this hazard

Cover TG 495

  • TG 495 measures the phototoxicity of a chemical in vitro, through a reactive oxygen species assay

Cover TG 248

  • TG 248 measures the capacity of a chemical to activate or inhibit thyroid function in a frog eleutheroembryo.

Also, the following Test Guidelines have been updated:

  • TG 203 (fish acute toxicity test),
  • TG 442C (in vitro skin sensitisation assay, covalent binding to protein),
  • TG 492 (in vitro eye irritation assay using reconstructed human eye corneal epithelium)
  • TG 431 (in vitro skin corrosion assay using reconstructed human epidermis)
  • TG 432 (in vitro 3T3 Neutral Red Uptake Phototoxicity test)
  • TG 439 (in vitro skin irritation assay using reconstructed human epidermis).

A set of supporting documents associated to these Test Guidelines will soon be published.


Access the Test Guidelines 

The OECD Guidelines are a unique tool for assessing the potential effects of chemicals on human health and the environment. Accepted internationally as standard methods for safety testing, the Guidelines are used by professionals in industry, academia and government involved in the testing and assessment of chemicals (industrial chemicals, pesticides, cosmetics, etc.). These Guidelines are regularly updated with the assistance of hundreds of national experts from OECD member countries.

Information on the Mutual Acceptance of Data (MAD) system, the full list of adopted Test Guidelines, the draft TGs under public commenting rounds, the draft Guidance and review documents are available on the Test Guidelines Programme


Publications in the Series of Testing and Assessment 

This Series includes publications related to testing and assessment of chemicals; some of them support the development of OECD Test Guidelines (e.g. validation reports, guidance documents, detailed review papers).


Test Guidelines that have been deleted or replaced by updated versions

The Test Guidelines that have been deleted should not be used for new testing. They are included here because it may be useful to consult them in the framework of the assessment of substances based on old study reports. The guaranties of Mutual Acceptance of Data (MAD) would not apply if these Test Guidelines were used for new testing.

In November 2012, the Joint Meeting of the Chemicals Committee and Working Party on Chemicals, Pesticides and Biotechnology decided on a transition period of 18 months, between the Council Decision and the effective deletion, for Test Guidelines that have been updated or deleted. For more information, please read the presentation: Update of Test Guidelines and the Mutual Acceptance of Data.

Further reading


Related Documents