The Isolated Chicken Eye (ICE) test method is an in vitro test method that can be used to classify substances as “ocular corrosives and severe irritants”. The ICE method uses eyes collected from chickens obtained from slaughterhouses where they are killed for human consumption, thus eliminating the need for laboratory animals. The eye is enucleated and mounted in an eye holder with the cornea positioned horizontally. The test substance and negative/positive controls are applied to the cornea. Toxic effects to the cornea are measured by a qualitative assessment of opacity, a qualitative assessment of damage to epithelium based on fluorescein retention, a quantitative measurement of increased thickness (swelling), and a qualitative evaluation of macroscopic morphological damage to the surface. The endpoints are evaluated separately to generate an ICE class for each endpoint, which are then combined to generate an Irritancy Classification for each test substance.
This Guidance Manual for the Testing of Manufactured Nanomaterials, is a “living document” and as such, it will be updated and amended in an iterative manner based upon knowledge accumulation as the testing programme and Dossier Development Plans (DDPs) work progresses.
These are the OECD Guidelines for the Testing of Chemicals: Testing and Assessment Series Monographs.
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OECD Guidance for Country Data Review Reports on Microbial Pest Control Agents (Monograph Guidance for Microbials), Series on Pesticides No 22 - Main document
Development of this siteThis website has been developed by the Issue Team on Sustainable Chemistry which is part of the OECD Environment, Health and Safety Programme. Germany served as a lead country in the development of this website. Purpose of the Sustainable Chemistry PlatformThis site was set up to facilitate information exchange, review of new developments and further elaboration of incentives for Sustainable Chemistry and to
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OECD Guidance for Industry Data Submissions for Microbial Pest Control Products and their Microbial Pest Control Agents (Dossier Guidance for Microbials), Series on Pesticides No. 23 - Main document
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This document comprises the compilation of output documents that have been produced duringthe course of the work of the project, and that can be useful to a wider audience, in addition to the WPMN.
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This document is the report of the OECD Workshop on Exposure Assessment and Exposure Mitigation, which was held on 20 October 2008, in Frankfurt, Germany. This report includes a summary of the plenary presentations as w
This conference, which took place in Paris on 15-17 July 2009, will significantly contribute to accelerating OECD’s current efforts for fostering “green” and innovation-led growth in nanotechnology.
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This document should be seen as a component of the overall work of the programme on the safety of manufactured nanomaterials, which is been developed by OECD’s WPMN.