Latest Documents


  • 23-July-2010

    English

    Test No. 442A: Skin Sensitization - Local Lymph Node Assay: DA

    The Local Lymph Node Assay: DA (LLNA: DA) is a non-radioactive modification to the LLNA method for identifying potential skin sensitizing test substances and measuring the proliferation of lymphocytes they induce in the auricular lymph nodes. The method, described in mouse (CBA/J strain), is based on measurement of the adenosine triphosphate (ATP) content by bioluminescence as an indicator of this proliferation. A minimum of four animals is used per dose group, with a minimum of three concentrations of the test substance, plus a concurrent negative control group and, if appropriate, a positive control group. The experimental schedule is during 8 days. The time from animal sacrifice to measurement of ATP should not exceed 30 min. The procedure from excision of lymph nodes to ATP measurement should be kept uniform for each animal and completed within 20 minutes. The luciferin/luciferase method is applied to measure the bioluminescence in Relative Luminescence Units (RLU). This study includes: measurements (weighing, RLU), and clinical daily observations. The results are expressed as the Stimulation Index (SI) obtained by calculation. The SI should be ¡Ý1.8 before further evaluation of the test material as a potential skin sensitizer is warranted.

  • 23-July-2010

    English

    Test No. 223: Avian Acute Oral Toxicity Test

    This Test Guideline describes procedures designed to estimate the acute oral toxicity of substances to birds, and it provides three testing options: (1) limit dose test, (2) LD50-slope test, and (3) LD50-only test. The LD50-slope and LD50-only options are sequential testing procedures. The test method selected will depend on whether or not a definitive median dose (LD50) and slope of the dose-response curve are both needed. The limit dose test is the preferred test when toxicity is expected to be low and lethality is unlikely at the limit dose. The limit dose should be adequate for assessment purposes, and it is usually 2000 mg/kg-bwt. Five or ten birds are tested at the limit dose in addition to a control group. The LD50-slope test is the preferred test when regulatory or other requirements determine that the slope of the dose-response curve and/or the confidence interval is required in addition to an estimate of the LD50. This is a 3- or 4-stage test with 24 or 34 birds in addition to a control group. The LD50-only test is the preferred test when regulatory or other requirements determine that only the median lethal dose is required but neither the slope of the dose response curve or the confidence interval for the LD50 is required. This may be the appropriate test to estimate a percentile of a species sensitivity distribution of LD50s and to provide information for product labelling purposes. This test has two stages, with 14 birds in addition to a control group.

    Software to be used with TG 223. Click here. Software not part of the Mutual Acceptance of Data.

  • 23-July-2010

    English

    Test No. 487: In Vitro Mammalian Cell Micronucleus Test

    The in vitro micronucleus test is a genotoxicity test for the detection of micronuclei in the cytoplasm of interphase cells. Micronuclei may originate from acentric chromosome fragments (i.e. lacking a centromere), or whole chromosomes that are unable to migrate to the poles during the anaphase stage of cell division. The assay detects the activity of clastogenic and aneugenic test substances in cells that have undergone cell division during or after exposure to the test substance. This Test Guideline allows the use of protocols with and without the actin polymerisation inhibitor cytochalasin B. Cytochalasin B allows for the identification and selective analysis of micronucleus frequency in cells that have completed one mitosis, because such cells are binucleate. This Test Guideline also allows the use of protocols without cytokinesis block provided there is evidence that the cell population analysed has undergone mitosis.

  • 7-July-2010

    English

    Current Developments/Activities on the Safety of Manufactured Nanomaterials

    This document provides information on current/planned activities related to the safety of manufactured nanomaterials in OECD member and non-member countries that attended at the 7th meeting of OECD’s Working Party on Manufactured Nanomaterials (Paris France, 7-9 July 2010).

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  • 7-July-2010

    English

    Guidance Manual for the Testing of Manufactured Nanomaterials: OECD Sponsorship Programme: First Revision

    The objective of this document, Guidance Manual for the Testing of Manufactured Nanomaterials, is to assist sponsors in the development of Dossier Development Plans (DDPs).

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  • 16-June-2010

    English, , 237kb

  • 7-June-2010

    English, , 4,766kb

    Report of the Expert Consultation on Scientific and Regulatory Evaluation of Organic Chemistry Mechanism-Based Structural Alerts for the Identification of DNA Binding Chemicals (part 2)

    This document is a report of the expert consultation held on 20 October 2009 with the aim to evaluate a set of structural alerts for estimating covalent binding of chemicals with DNA.

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  • 7-June-2010

    English, , 465kb

    Report of the Expert Consultation on Scientific and Regulatory Evaluation of Organic Chemistry Mechanism-Based Structural Alerts for the Identification of DNA Binding Chemicals (part 1)

    This document is a report of the expert consultation held on 20 October 2009 with the aim to evaluate a set of structural alerts for estimating covalent binding of chemicals with DNA.

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  • 1-June-2010

    English

    OECD Pov and LRTP Screening Tool

    A software in a spreadsheet format containing multimedia chemical fate models to estimate overall persistence and long-range transport potential.

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  • 1-June-2010

    English, , 170kb

    The Pov and LRTP Screening Tool Brochure

    Estimating overall persistence and long-range transport potential of organic chemicals.

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