The biotransformation of radiolabelled [test material] was studied in (a) – (texture) soil(s) (pH --, organic carbon -- %) from [country name] for – d under aerobic conditions in dark at – ΊC, and x % moisture. [test material] was applied at the rate of – mg a.i./kg soil, equivalent to – g a.i./ha.  The experiment was conducted in accordance with the – (specify the Guidelines followed), and in compliance with the – GLP standards (specify the GLP standards followed e.g., OECD-GLP).  The test system consisted of (e.g.:  Erlenmeyer flasks) attached/not attached with traps for the collection of CO2 and volatile organics.  Samples were analysed at 0, x1, x2, -- and Xn days of incubation.  The soil samples were extracted with – (specify the solvent and/or method used), and the [test material] residues were analysed by – [TLC/HPLC – (detector)/GC-(detector)].  Identification of the transformation  products was done by – (e.g.:  GC-MS, co-chromatography etc.).

 

Material balance was – (mean ± s.d.) % of the applied amount.  The concentration of the parent compound decreased from -- % of the applied amount at day 0, to -- % of the applied at the end of study period.  The half-life (lives)/DT50 (50% decline time) [test material] in aerobic soil was/were – and – days.  [Briefly discuss the rate of transformation of the parent compound].  {If there was no transformation state – No biotransformation occurred.  If there was transformation, incorporate the following information].

 

 

The major transformation products detected were – (Code and chemical nomenclature), with maximum concentrations of – and -- % of the applied amount, observed on the –th and –th day of incubation, respectively.  The corresponding concentrations at the end of the study period were – and -- % of the applied amount, respectively.  {Specify if no major transformation products were detected).  The minor transformation products were –and – formed at a maximum of – to -- % of the applied amount.  The total unidentified radioactivity was – to -- % (range) of the applied amount.  Extractable [14C]residues decreased from --% of the applied amount at day 0 to -- % of the applied at the end of study period.  Non-extractable [14C]residues increased from -- % of the applied amount at day 0 to -- % of the applied at the end of the incubation period.  At study termination, evolved 14CO2 and volatile organics were -- % and -- % of the applied amount, respectively.

[If sterile treatments were used, compare the transformation of the parent, including the type and amount of transformation products formed, and the extent of non-extractable residue formation, with that of the non-sterile treatments].

 

Describe the pathway of aerobic biotransformation of [test material] in soil.

 

[Briefly describe the results of the supplementary experiment, if any.]