The effect of {test material} on the seed germination, seedling emergence and vegetative vigour of monocot {insert scientific and common names of tested species} and dicot {insert scientific and common names of tested species} crops was studied at {nominal/measured} concentrations of {0, x1, x2, …xn mg a.i./kg soil or a.i./ha}.  The growth medium used in the seedling emergence and vegetative vigour test was natural soil (texture, pH, organic carbon %)/artificial soil (specify)/nutrient solution (specify). Each seed with a radicle of …mm or longer after … days was considered germinated.  On day … and .. , the surviving plants per pot were recorded and cut at soil level for measuring the plant height and dry weight in the seedling emergence and vegetative vigour test, respectively.

 

Seed germination was/ was not affected by the treatment of [test material].  The % growth inhibition in seed germination in the treated species as compared to the control ranged from … to …  The most sensitive monocot species was … with a EC25 of … mg a.i./kg soil or a.i./ha.  The most sensitive dicot species was … with a EC25 of … mg a.i./kg soil or a.i./ha.  The NOEC based on the seed germination was … mg a.i./kg soil or a.i./ha.

 

The % seedling emergence was significantly affected in … [indicate the crops].  The % inhibition in seedling emergence in the treated species as compared to the control ranged from … to … The most sensitive monocot species in the seedling emergence test was … with a EC25 of … mg a.i./kg soil or a.i./ha.  The most sensitive dicot species was … with a EC25 of … mg a.i./kg soil or a.i./ha.  The NOEC based on the seedling emergence was … mg a.i./kg soil or a.i./ha.

 

In the vegetative vigour test, the plant dry weight and plant height were/were not affected by [test material] treatment.  The NOEC values based on the plant growth (length or dry weight)/ or other end points was … mg a.i./kg soil or a.i./ha.  The most sensitive monocot species in the vegetative vigour test was … with a EC25 of … mg a.i./kg soil or a.i./ha.  The most sensitive dicot species was … with a EC25 of … mg a.i./kg soil or a.i./ha.  The most sensitive end point in the vegetative vigour test was NOEC (… mg a.i./kg soil or a.i./ha) based on the phytotoxicity symptoms/dry weight in – (specify the crop).

 

The following abnormalities were noted:  (list abnormalities that may include abnormal seedling development or appearance, lesion, swelling, loss of turgor, discoloration, root shape or size).  [If toxicity and abnormalities were not observed, state “There were no compound related phytotoxic effects.”]

 

Maximum Labeled Rate:  {…………. lb a.i./A or g a.i./ha}

 

Results Synopsis

                              Seed germination       Seedling emergence        Vegetative vigour

Monocot

EC25/IC25:              (lb a.i./A or g a.i./ha)

EC50/IC50:              (lb a.i./A or g a.i./ha)

NOEC or EC05:     (lb a.i./A or g a.i./ha)

Probit Slope:

95% C.I.:

Most sensitive monocot:

Most sensitive parameter:

 

Dicot

EC25/IC25:              (lb a.i./A or g a.i./ha)

EC50/IC50:              (lb a.i./A or g a.i./ha)

NOEC or EC05:     (lb a.i./A or g a.i./ha)

Probit Slope:

95% C.I.:

Most sensitive dicot:

Most sensitive parameter:

 

This toxicity study is classified as [enter acceptability classification e.g. acceptable/ un acceptable/supplementary) and {satisfies/does not satisfy} the guideline requirement for {indicate study type} toxicity study.