The biotransformation of [test material] was studied in filtered/unfiltered pond/river water (pH --, dissolved organic carbon -- %) from [country name] for d under aerobic conditions in dark at -- ΊC. [test material] was applied at the rate of mg a.i./L, equivalent to g a.i./ha. The experiment was conducted in accordance with the (specify the Guidelines followed), and in compliance with the GLP standards (specify the GLP standards followed; e.g., OECD-GLP). The test system consisted of (e.g.: Erlenmeyer flasks), attached/not attached with traps for the collection of CO2 and volatile organic compounds. Samples were analysed at 0, x1, x2, --, and Xn days of incubation. The water samples were extracted with (specify the solvent and/or method used; indicate if the water samples were not extracted), and the [test material] residues were analysed by [TLC/HPLC (detector)/GC (detector)]. Identification of the transformation products was done by (e.g.: GC-MS, co-chromatography etc.).
Material balance was (mean ± s.d.) % of the applied amount. The concentration of parent compound decreased from -- % at day 0 to -- % of the applied amount at the end of study period. The half-life/DT50 (50% decline time) of [test material] in aerobic water was days. [Briefly discuss the rate of transformation of the parent]. [If there was no transformation state No biotransformation occurred. If there was transformation, incorporate the following information.]
The major transformation products detected were (Code and chemical nomenclature), with maximum concentrations of and -- % of the applied amount, observed on the th and th day of incubation, respectively. The corresponding concentrations at the end of the study period were and -- % of the applied amount, respectively. (Specify if no major transformation products were detected). The minor transformation products were and formed at a maximum of to -- % of the applied amount. At study termination, evolved 14CO2 and volatile organic compounds accounted for -- % and -- % of the applied amount, respectively. The unidentified radioactivity in the study was -- % (range) of the applied amount. The pathway of aerobic biotransformation of [test material] in water was (describe the pathway).
[If sterile treatments were used, compare the transformation of the parent, including the type and amount of transformation products formed, with that of the non-sterile treatments.]
[Briefly describe the results of the supplementary experiment, if any.]