The biotransformation of [test material] was studied in filtered/unfiltered pond/river water (pH --, dissolved organic carbon -- %) from [country name] for – d under aerobic conditions in dark at -- ΊC.  [test material] was applied at the rate of – mg a.i./L, equivalent to – g a.i./ha.  The experiment was conducted in accordance with the – (specify the Guidelines followed), and in compliance with the – GLP standards (specify the GLP standards followed; e.g., OECD-GLP).  The test system consisted of – (e.g.:  Erlenmeyer flasks), attached/not attached with traps for the collection of CO2  and volatile organic compounds.  Samples were analysed at 0, x1, x2, --, and Xn days of incubation.  The water samples were extracted with – (specify the solvent and/or method used; indicate if the water samples were not extracted), and the [test material] residues were analysed by – [TLC/HPLC – (detector)/GC – (detector)].  Identification of the transformation products was done by – (e.g.:  GC-MS, co-chromatography etc.).

 

Material balance was – (mean ± s.d.) % of the applied amount.  The concentration of parent compound decreased from -- % at day 0 to -- % of the applied amount at the end of study period.  The half-life/DT50 (50% decline time) of [test material] in aerobic water was – days.  [Briefly discuss the rate of transformation of the parent].  [If there was no transformation state – No biotransformation occurred.  If there was transformation, incorporate the following information.]

 

The major transformation products detected were – (Code and chemical nomenclature), with maximum concentrations of – and -- % of the applied amount, observed on the –th and –th day of incubation, respectively.  The corresponding concentrations at the end of the study period were – and -- % of the applied amount, respectively.  (Specify if no major transformation products were detected).  The minor transformation products were – and – formed at a maximum of – to -- % of the applied amount.  At study termination, evolved 14CO2 and volatile organic compounds accounted for -- % and -- % of the applied amount, respectively.  The unidentified radioactivity in the study was -- % (range) of the applied amount.  The pathway of aerobic biotransformation of [test material] in water was – (describe the pathway).

 

[If sterile treatments were used, compare the transformation of the parent, including the type and amount of transformation products formed, with that of the non-sterile treatments.]

 

[Briefly describe the results of the supplementary experiment, if any.]