The aerobic biotransformation of [test material] was studied in pond or river water/sediment system (water pH --, dissolved organic carbon -- %; sediment texture --, pH --, organic carbon -- %) from [country name] for d in dark at -- ΊC. [test material] was applied at the rate of mg a.i./L. The sediment/water ratio used was --. The experiment was conducted in accordance with the (the Guidelines followed), and in compliance with the GLP standards (specify the GLP standards followed, e.g., OECD-GLP). The test system consisted of (e.g. Erlenmeyer flasks) attached/not attached with traps for the collection of CO2 and organic volatile compounds. Samples were analyzed at 0, x1, x2, -- and Xn days of incubation. The water samples were extracted with (solvent and/or method used; indicate, if not extracted), and the sediment samples were extracted with (solvent and/or method used). [Test material] residues were analysed by [TLC/HPLC (detector)/GC-(detector)]. Identification of the transformation products was done by (e.g.: GC-MS, co-chromatography etc.).
The mean total recovery of radiolabelled material was and -- % mean (mean ± std) of the applied in water and sediment, respectively. The concentration of [parent] in water decreased from -- % at day 0, to -- % of the applied at study termination. The concentration of [parent] in the sediment increased from -- % at day 0 to --% of the applied, at the end of the study period. On day/at test termination, -- % of the applied radioactivity was partitioned from water to sediment.
The major transformation products detected in water were (Code and chemical nomenclature), with maximum concentrations of and -- % of the applied amount, observed on the th and th day of incubation, respectively. The corresponding concentrations in water at the end of the study period were and -- % of the applied amount, respectively. The major transformation products detected in sediment were (Code and chemical nomenclature), with maximum concentrations of and -% of the applied amount, observed on the th and th day of incubation, respectively. The corresponding concentrations in sediment at the end of the study period were and -- % of the applied amount, respectively. (Specify if no major transformation products were detected). The minor transformation products in water were and formed at a maximum of to -- % of the applied amount. The minor transformation products in sediment were and formed at a maximum of to -- % of the applied amount. The unidentified 14C during the study was -- % (range) of the applied amount.
Extractable [14C]residues in sediment decreased from -- % at day 0, to -- % of the applied amount at the end of incubation period. Non-extractable [14C] residues in sediment increased from -- % at day 0, to -- % of the applied amount, at study termination. At the end of the study, -- % and --% of the recovered radioactivity was present as CO2 and organic volatile compounds, respectively. [If sterile treatments are used, compare the transformation in sterile and non-sterile treatments].
The half-life (lives)/50% decline times (DT50) of [test material] in aerobic water, sediment and in the entire system was/were --, --, and days, respectively.
Describe the pathway of aerobic biotransformation of [test material] in water-sediment system.
[Briefly describe the results of the supplementary experiments, if any].