The phototransformation of 14C-[test material] was studied on [texture] soil [pH --, organic carbon --%] from [country name] at – mg a.i./kg soil for – d at --ΊC and --% moisture.  The experiment was conducted in accordance with the – (specify the Guideline followed), and in compliance with the – GLP standards [specify the GLP standards followed].  [test material] was applied on the soil surface by spraying or other method.  The treated samples were irradiated under natural sunlight (location; latitude; longitude; light intensity; sunlight hours) or by continuous irradiation using – (type of lamp; wavelength/filter used).  Test vessels were connected/not connected to traps for the collection of CO2 and organic volatiles.  Samples were taken at 0, x1, --, and xn days for the determination of the parent compound and transformation products.  The soil samples were extracted with – (specify the solvent and/or method used), and the [test material] residues were analysed by – [TLC/HPLC – (detector)/GC-(detector)].  Identification of the transformation products was done by – (e.g.:  GC-MS, co-chromatography).

 

The mass balance was – and -- % (mean ± s.d.) in the dark and irradiated samples, respectively.  At test termination, --% of the applied 14C remained as the parent in the dark samples.  [If there was transformation, incorporate the following information, otherwise state – No transformation occurred in the dark samples].  The transformation products identified in the dark samples were – and – formed at a maximum of – to -- % of the applied amount.  At study termination, the CO2 and volatile organic compounds in the dark samples amounted to – and --% of the applied amount, respectively.  [Indicate if any of the transformation products in the dark were major or decreased after reaching the maximum.]

 

In the irradiated samples, concentration of the parent compound decreased from -- % at day 0 to --% of the applied amount at test termination.  [If there was transformation, incorporate the following information, otherwise state – No transformation occurred in the irradiated samples].  The major transformation product(s) was/were – [code name(s) and chemical nomenclature], with maximum concentrations of – and -- % of the applied amount, observed on the –th and –th day of incubation, respectively.  At study termination, the corresponding concentrations were – and -- % of the applied amount, respectively.  The minor transformation product(s) in the irradiated samples was/were – {code name(s) and chemical nomenclature} formed at a maximum of – to -- % of the applied amount.  [Indicate if any of the transformation products decreased after reaching the maximum.]   In irradiated samples, at the end of the study, the evolved CO2 and volatile organic compounds amounted to – and -- % of the applied amount, respectively.  (Indicate if the volatiles were not formed). [Compare the transformation products formed in the dark and irradiated samples, and, identify and quantify the products that are formed by phototransformation only.]

 

Extractable [14C] residues decreased from – and -- % of the applied amount at day 0, to – and -- % of the applied amount at test termination, in the dark and irradiated samples, respectively.  In the irradiated samples, non-extractable [14C] residues increased from -- % of the applied amount at day 0, to -- % of the applied, at the end of the study.  Non-extractable residues in the dark samples was -- % of the applied amount at day 0, and -- % of the applied amount at test termination.  Briefly describe the phototransformation pathway.

 

The half-life (lives)/DT50 (50% decline time) of [test material] in the dark and irradiated samples was/were – and –d, respectively.  The half-life (lives)/DT50 for phototransformation was – d [difference between irradiated and dark samples].

 

[Briefly describe the results of the supplementary experiments, if any]