The aqueous phototransformation of radiolabelled [test material] was studied at -- ΊC in sterile aqueous (type of buffer; e.g., acetate) buffer solutions at pH x at an initial concentration of mg a.i./L under natural sunlight (location; latitude; light intensity; sunlight hours) or by continuous irradiation using (type of lamp and wavelength/filter used) for d. The experiment was conducted in accordance with the (specify the Guideline followed), and in compliance with the GLP standards (specify the GLP standards followed; e.g., OECD-GLP). Test vessels were connected/not connected to traps for the collection of CO2 and organic volatiles. Samples were analysed at 0, x1, --, and xn days by extracting with (specify the solvent and/or method used; indicate, if the samples were not extracted), and the [test material] residues were analysed by [TLC/HPLC - (detector)/GC-(detector)]. Identification of the transformation products was done by (e.g.: GC-MS, co-chromatography etc.).
The mass balance was and -- % of the applied amount (mean ± s.d. or range) in the dark and irradiated samples, respectively. At test termination -- % of the applied radioactivity remained as the parent in the dark samples. [If there was transformation, incorporate the following information, otherwise state No transformation occurred in the dark samples]. The transformation products identified in the dark samples were and formed at a maximum of to -- % of the applied amount. [Indicate if any of the transformation products in dark samples were major or the concentration decreased after reaching the maximum]. At study termination, the evolved CO2 and volatile organic compounds in the dark samples amounted to and -- % of the applied amount, respectively. [Indicate if the volatiles were not formed].
In the irradiated samples, the concentration of the parent compound decreased from -- % at day 0 to -- % of the applied amount at study termination. [If there was transformation, incorporate the following information, otherwise state No transformation occurred in the irradiated samples.] The major transformation products detected in the irradiated samples were (Code and chemical nomenclature), with maximum concentrations of and k% of the applied amount observed on the th and th day of incubation/at study termination, respectively. [Indicate if the concentration of major transformation products decreased after reaching the maximum]. [Specify if no major transformation products were detected]. The minor transformation products in the irradiated samples were and formed at a maximum of to -- % of the applied amount, respectively. At study termination, in the irradiated samples, the evolved CO2 and volatile organics amounted to and -- % of the applied amount, respectively. [Indicate if the volatiles were not formed.] The total unidentified radioactivity was and -- % of the applied amount in the dark and irradiated samples, respectively.
[Compare the transformation products formed in the dark and irradiated samples, and identify and quantify the products that are formed by phototransformation only.] Briefly describe the phototransformation pathway.
The half-life (lives)/DT50 (50% decline time) of [test material] in the dark and irradiated samples was/were and d, respectively. The half-life/DT50 for phototransformation was d (difference between the irradiated and dark samples).
Report the predicted environmental phototransformation half-life, if provided. [e.g. The predicted environmental photolytic half-life, derived from the measured half-life in laboratory under artificial lamp, was calculated to be d for latitude midday midsummer/other at (city name)]
[Briefly describe the results of the supplementary experiments, if any]