The aqueous phototransformation of radiolabelled [test material] was studied at -- ΊC in sterile aqueous – (type of buffer; e.g., acetate) buffer solutions at pH x at an initial concentration of – mg a.i./L under natural sunlight (location; latitude; light intensity; sunlight hours) or by continuous irradiation using (type of lamp and wavelength/filter used) for – d.  The experiment was conducted in accordance with the – (specify the Guideline followed), and in compliance with the – GLP standards (specify the GLP standards followed; e.g., OECD-GLP).  Test vessels were connected/not connected to traps for the collection of CO2 and organic volatiles.  Samples were analysed at 0, x1, --, and xn days by extracting with – (specify the solvent and/or method used; indicate, if the samples were not extracted), and the [test material] residues were analysed by – [TLC/HPLC - (detector)/GC-(detector)].  Identification of the transformation products was done by – (e.g.:  GC-MS, co-chromatography etc.).

 

The mass balance was – and -- % of the applied amount (mean ± s.d. or range) in the dark and irradiated samples, respectively.  At test termination -- % of the applied radioactivity remained as the parent in the dark samples.  [If there was transformation, incorporate the following information, otherwise state – No transformation occurred in the dark samples].   The transformation products identified in the dark samples were – and – formed at a maximum of – to -- % of the applied amount. [Indicate if any of the transformation products in dark samples were major or the concentration decreased after reaching the maximum].  At study termination, the evolved CO2 and volatile organic compounds in the dark samples amounted to – and -- % of the applied amount, respectively.  [Indicate if the volatiles were not formed].

 

In the irradiated samples, the concentration of the parent compound decreased from -- % at day 0 to -- % of the applied amount at study termination.  [If there was transformation, incorporate the following information, otherwise state – No transformation occurred in the irradiated samples.]  The major transformation products detected in the irradiated samples were – (Code and chemical nomenclature), with maximum concentrations of – and – k% of the applied amount observed on the – th and –th day of incubation/at study termination, respectively.  [Indicate if the concentration of major transformation products decreased after reaching the maximum].  [Specify if no major transformation products were detected].  The minor transformation products in the irradiated samples were – and – formed at a maximum of – to -- % of the applied amount, respectively.  At study termination, in the irradiated samples, the evolved CO2 and volatile organics amounted to – and -- % of the applied amount, respectively.   [Indicate if the volatiles were not formed.]  The total unidentified radioactivity was – and -- % of the applied amount in the dark and irradiated samples, respectively.

 

[Compare the transformation products formed in the dark and irradiated samples, and identify and quantify the products that are formed by phototransformation only.]  Briefly describe the phototransformation pathway.

 

The half-life (lives)/DT50 (50% decline time) of [test material] in the dark and irradiated samples was/were – and – d, respectively.  The half-life/DT50 for phototransformation was – d (difference between the irradiated and dark samples).

 

Report the predicted environmental phototransformation half-life, if provided.  [e.g. The predicted environmental photolytic half-life, derived from the measured half-life in laboratory under artificial lamp, was calculated to be – d for – latitude midday midsummer/other at – (city name)]

 

[Briefly describe the results of the supplementary experiments, if any]