Hydrolysis of radiolabelled [test material] at – mg a.i./L was studied in the dark at --ΊC in sterile aqueous buffered solutions at pH 5 (type of buffer; e.g. , acetate buffer), pH 7 (type of buffer) and pH 9 (type of buffer) for – days.  The experiment was conducted in accordance with the – (specify the Guideline followed), and in compliance with the – GLP standards (specify the GLP standards followed; e.g., OECD-GLP).  Samples were analysed at 0, x1, --, and xn days by extracting with – (specify the solvent and/or method used; indicate, if the samples were not extracted), and the [test material] residues were analysed by – [TLC/HPLC – (detector)/GC-(detector)].  Identification of the transformation products was done by – (e.g.:  GC-MS, co-chromatography, etc.).

 

The radioactivity balance was – (mean ± s.d.), -- (mean ± s.d.), and -- % (mean ± s.d.) of the applied at pH 5, pH 7, and pH 9, respectively.  At test termination, the concentration of the parent compound decreased from -- % at day 0, to --% of the initial at pH 5, from – to --% of the initial at pH 7, and from – to --% of the initial at pH 9. [If there was no transformation of the parent, state that – most of the applied 14C was associated with the parent compound at test termination.  If there was transformation, incorporate the following].  At pH 5, the major transformation products detected were – (Code and chemical nomenclature), with maximum concentrations of – and -- % of the applied, observed on the –th and –th day of incubation/at test termination, respectively.  At pH 7, the major transformation products detected were – (Code and chemical nomenclature), with maximum concentrations of – and --% of the applied, observed on the –th and –th day of incubation/ at test termination, respectively.  At pH 9, the major transformation products detected were – (Code and chemical nomenclature), with maximum concentrations of – and -- % of the applied amount, observed on the –th and –th day of incubation/at test termination, respectively.  [Specify if no major transformation products were detected in any of the pHs].  The minor transformation product(s) was/were – {Code name(s) and chemical nomenclature}, and formed at a maximum of --, -- and -- % of the applied at pH 5, pH 7, and pH 9, respectively. [indicate if the concentration of the transformation products decreased after reaching the maximum].  At the end of the study, the evolved CO2 was --, --, and -- % of the applied at pH 5, pH 7, and pH 9, respectively.  The total volatile organics at test termination was --, --, and -- % of the applied amount at pH 5, pH 7, and pH 9, respectively (Indicate if the volatiles were not formed).  The unidentified radioactivity was --, --, and -- % of the applied amount at pH 5, pH 7, and pH 9, respectively.

 

The half-life (lives)/DT50 (50% decline times) of [test material] were--, --, and –d at pH 5, pH7, and pH 9, respectively.

 

[Briefly describe the results of the supplementary experiments, if any]

 

This study is classified {acceptable or unacceptable/supplemental} and [satisfies/does not satisfy] the guideline requirement for hydrolysis study.