Table #:  Natural Killer Cell Assay

 

Test Group (n = #)

 

Effector:Target Ratio (b) eg.,

 

LU/107 cells (c)

(Specific activity)

 

LU/spleen (c)

(Total activity)

 

6.25:1

 

12.5:1

 

25:1

 

50:1

 

100:1

 

200:1

 

Male

 

Control

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Dose 1

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Dose 2

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Dose 3

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Positive Control (name of substance)

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Female

 

Control

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Dose 1

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Dose 2

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Dose 3

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Positive Control (name of substance)

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

b Values are expressed as percent cytotoxicity

c Values expressed as lytic unit (LU) were LU is defined as the number of splenocytes required to kill 10% of the target cells

Effector cells:  NK cells.  Target cells:  YAC-1 lymphoma cells

*  Significantly different (p <0.05) from the control.

** Significantly different (p <0.01) from the control.