Table #: DNT  Study design  – Animal assignment

Experimental Parameter

0

LDT

MDT

HDT

Maternal Animals

No. of maternal animals assigned

 

 

 

 

FOB (GD #, #; LD #, #)

10

10

10

10

Offspring

Detailed clinical/FOB (PND #, #, #, #, #, #)

10/sex

10/sex

10/sex

10/sex

Motor activity (PND #, #, #, #)

10/sex

10/sex

10/sex

10/sex

Auditory startle habituation (PND #, #)

10/sex

10/sex

10/sex

10/sex

Learning and memory (PND #, #)

10/sex

10/sex

10/sex

10/sex

Brain weight PND 11

PND 60

10/sex

10/sex

10/sex

10/sex

10/sex

10/sex

10/sex

10/sex

Neuropathology PND 11

PND 60

 10/sex

 10/sex

 10/sex

 10/sex

 10/sex

 10/sex

 10/sex

 10/sex

Blood  cholinesterase determination [if applicable]

 # /sex

 # /sex

 # /sex

 # /sex

Brain cholinesterase determination [if applicable]

 # /sex

 # /sex

 # /sex

 # /sex